Characterization of a Novel Miniature Cell Culture Device
- Paper number
IAC-06-A1.P.2.01
- Author
Ms. Sandra Geffert, University of Houston, United States
- Coauthor
Dr. Stanley J. Kleis, United States
- Year
2006
- Abstract
Recent advancements in the field of microfluidics have generated much interest in the advent of a miniaturized cell culture device. In this study, we developed a novel continuous miniature culture system (<1mL) for the long-term cultivation of both anchorage dependent and suspension cells, either prokaryotic or eukaryotic in type, for both 1g and microgravity applications. The miniature culture system is a novel device that may advance the development of micro analytical remote monitoring tools such as biological sentinels, biosensors, and lab-on-a-chip. Integrating the autonomous miniature culture system with a micro-analytical device provides one with a powerful biological tool. Cells can be cultured long term, harvested, and released directly into an analytical tool without the need for human interaction through fluid dynamic manipulations. This work characterizes the miniature bioreactor system through numerical and time lapse microscopy studies. Selective cell removal capabilities are demonstrated and procedures are outlined for the long term use of the mini-culture system.
- Abstract document
- Manuscript document
IAC-06-A1.P.2.01.pdf (🔒 authorized access only).
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