UV biological sensor versus MED and its potential applications in space studies involving the UV radiation effects on life systems
- Paper number
IAC-08.A1.6.5
- Author
Mr. Pabulo Henrique Rampelotto, Southern Regional Space Research Center - CRS/CIE/INPE - MCT, Brazil
- Coauthor
Mr. Cristiano Sarzi Machado, Southern Regional Space Research Center - CRS/CIE/INPE - MCT, in collaboration with the Space Science Laboratory of Santa Maria LACESM/CT/UFSM, Brazil
- Coauthor
Dr. Marcelo Barcellos daRosa, Southern Regional Space Research Center - CRS/CIE/INPE - MCT, Brazil
- Coauthor
Dr. Nelson Jorge Schuch, Southern Regional Space Research Center - CRS/CIE/INPE - MCT, Brazil
- Coauthor
Dr. Nobuo Munakata, Rikkyo University, Japan
- Year
2008
- Abstract
The solar UV radiation (UVR) is the most deleterious factor for biological systems in the space environment due to the high capacity of its shorter wavelengths to induce DNA damages. Consequently, the focus of recent studies has been to understand how is possible to adapt concepts and technologies of biological UV dosimetry developed under terrestrial conditions to space environment or others planetary surfaces. At the INPE’s Southern Space Observatory (29.4°S, 53.8°W), South of Brazil, a biological dosimeter based in the spore inactivation doses (SID) of Bacillus subtilis strain TKJ6312, has been used to monitor the UVR and compared with MED (Minimal Eritematose Dose, which simulate the human sensitivity to UVR) data, obtained by Brewer Spectrophotometers. Due the Nucleotide Excision Repair (NER) and Spore Photoproduct Lyase (SP lyase) repair mechanisms, the spores acquire a sensibility to UVR and presents characteristically resistance for extreme environment conditions. The biological dosimetry fulfills the criterions established by BIODOS project from the European Commission to be applied as UV-biosensor including its simplicity, facility of use and transport, long term storage and action spectrum with a good resolution. The published first laboratory results show that the SID action spectrum profile agrees with the MED action spectrum. Similar results were also found in field experiments for minutes and days. On the other hand, the correlated long term behaviors of both measures, under different and adverse climatological conditions still remain unknown. Considering a long monitoring period, since 2000, a high correlation index about 0.9 of monthly expositions and compared with Brewer’s MED measurements indicate its application for long-term period. Furthermore, spore’s data analyses from other sites around the world are in agreement for UVR season variation observed in the literature, from equatorial to higher latitudes sites. The simplicity, robustness and high resistance of bacterial spores under extreme environment conditions makes the biosensor an applicable and potential biological tool in space and others planetary surfaces.
- Abstract document
- Manuscript document
IAC-08.A1.6.5.pdf (🔒 authorized access only).
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