Expression of ICAM-1 and VCAM-1 in human umbilical vein endothelial cells under simulated microgravity
- Paper number
IAC-09.A1.7.8
- Author
Mr. zhang yu, China
- Coauthor
Prof. Sang Chen, China
- Coauthor
Mr. Han Guanjun, China
- Coauthor
Mr. Ma Xiao, China
- Coauthor
Dr. Fengyuan Zhuang, Beihang University, China
- Year
2009
- Abstract
By using FACS-analysis and immunohistochemical staining, we have studied the constitutive and TNF-$\alpha$ induced expression of ICAM-1 and VCAM-1 in human umbilical vein endothelial cells (HUVECs) exposed to simulated microgravity (SG). Untreated HUVECs did not contain detectable amounts of VCAM-1, but were ICAM-1 positive. After 5min or 30 min of clinorotation by RCCS (rotary cell culture system, NASA) at 15rpm, ICAM-1 expression on the cell membrane increased, while no significant change in VCAM-1 expression can be found. However, SG treatment for cultured cells activated by TNF-$\alpha$ at a low (10ng/ml) concentration, reduced VCAM-1 expression but caused an increase of ICAM-1 expression. With 24h or 48h SG treatment, ICAM-1 expression on the membrane of HUVECs without TNF-$\alpha$ stimulation increased, while VCAM-1 expression significantly decreased; and same trend was observed for the group with 24h or 48h SG treatment and TNF-$\alpha$ (10ng/ml) stimulation. ICAM-1 clustering of SG group with TNF-$\alpha$ (10ng/ml) were observed, as compared with the group with TNF-$\alpha$ stimulation but without SG treatment by immunofluorescence and laser confocal microscopy. Both ICAM-1 and VCAM-1 expression on endothelial cells(EC) is a pivot for WBC transendothelial migration(TEM). Our results showed different trends of ICAM-1 and VCAM-1 expression with SG treatment，which suggest that the signal transduction pathways are different for ICAM-1 and VCAM-1 expression under SG treatments. How do these different trends affect the immunology function is waiting for a deep investigation. {\bf Reference:} [1] Buravkova L, Romanov, Y, Rykova M,Grigorieva O, Merzlinkia N. Cell-to-cell interactions in changed gravity: round-based and flight experiments[J]. Acta Astronaut. 2005(2-8), 57, 67-74. [2] Paul JM, Christy JP, Karen AA, {\it et al}. The effects of spaceflight on adrenergic receptors and agonists and cell adhesion molecule expression[J]. Journal of Neuroimmunology. 2002, 132(1-2): 173-179. [3] Romanov YA, Buravkova LB, Rykova MP, {\it et al}. Expression of cell adhesion molecules and lymphocyte-endothelium interaction under simulated hypogravity in vitro[J]. Journal of Gravityand Physiology. 2001, 8(1): 5-8. [4] Stowe RP , Mehta SK, Ferrando AA, {\it et al}. Immune responses and latent herpes virus reactivation in spaceflight1 Aviat Space Environ Med[J]. 2001, 72 ( 10) : 8842-8911. [5] Horwitz AR, Parsons JT. Cell migration-movin`on[J]. Science. 1999, 286(5442): 1102-1103.- Abstract document
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