uvb induced damage and repair kinetics in human lymphocytes
- Paper number
IAC-10.A1.4.13
- Author
Mrs. Cuilin Cheng, Harbin Institute of Technology, China
- Coauthor
Mr. Zhao Haitian, Harbin Institute of Technology, China
- Coauthor
Prof. Wang Zhengyu, Harbin Institute of Technology, China
- Coauthor
Mrs. Lei Yao, China
- Year
2010
- Abstract
We have studied the dose - effect relationship of DNA damage and radiation intensity, and damage-repair kinetics in human lymphocytes induced by the direct effect of UVB (297nm and 302nm). The yields of DNA lesion were detected at the single cell level with the alkaline comet assay. Cell damage were measured at various incubation steps (0min, 10min, 20min, 40min, 60min, 90min, 120min, 180min, 240min, 24h) after four dose of UVB irradiation (OJ/m2, 5J/m2, 10J/m2, 20J/m2), the result showed that the DNA repair was not immediate effect. TDNA% increased gradually and then dropped rapidly with the prolonging incubation time after UVB irradiation. The number of breaks reached the peak at about 60min, and after 4h it showed a stable tendency but not a complete repair. At 24h of incubation, secondary DNA lesion aggravated at high dosages of radiation. TDNA\% of human lymphocytes at 20J/m2 irradiation was observed a significant decline, and cell has 50% the survival rate measured by MTS assay. Repair kinetics was analyzed by mathematical approach. The coefficients of NER kinetic functions in four doses have been compared by t-test and some appeared to be very different with relatively wide S.D. This result determines 24h after UVB irradiation should be the appropriate time for detecting the effect of new anti-radiation materials.
- Abstract document
- Manuscript document
(absent)