PKC and cell cytoskeleton in human umbilical vein endothelial cells under simulated microgravity
- Paper number
IAC-10.A1.7.4
- Author
Mr. Yu Zhang, Beihang University, China
- Coauthor
Prof. Sang Chen, China
- Coauthor
Dr. Fengyuan Zhuang, Beihang University, China
- Year
2010
- Abstract
Background: In our previous study, ICAM-1 expression on HUVECs increased after simulated microgravity, same results were found in parabolic flight. Morphology changes of F-actin and clustering of ICAM-1 on cell membrane were also observed. To investigate ICAM-1 downstream signal pathways in endothelial cells (ECs) under microgravity, we utilized rotating cell culture system(NASA) to culture ECs and activated by TNF-α. Phosphorylation of PKC-α and Src were detected by western blotting. We also analyze α-tubulin and β-actin content change after simulated microgravtiy. PKC-α phosphorylation was inhibited after 5min simulated microgravtiy, and significant inhibition was detected after 1h and 24h. However, α-tubulin and β-actin have no remarkable change after same time, which suggested cytoskeleton had a distribution change but no content change in short-term simulated microgravity. Otherwise, simulated microgravity induced PKC isoform expression in human lymphocytes, which prompted different PKC subunits had a different response to gravity change. All these need further research. Reference: [1] Buravkova L, Romanov Y, Rykova M, et al. Cell-to-cell interactions in changed gravity: round-based and flight experiments. Acta Astronaut. 2005, 57: 67-74. [2] Ullrich O, Huber K, Lang K. Signal transduction in cells of the immune system in microgravity. Cell Commun. Signal. 2008, 6: 9. [3] Infanger M, Ulbrich C, Baatout S, et al. Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells. J Cell Biochem, 2007, 101(6):1439-1455. [4] Carlsson S.I, Bertilaccio M.T, Ballabio E, et al. Endothelial stress by gravitational unloading: effects on cell growth and cytoskeletal organization. Biochim Biophys Acta, 2003, 1642(3):173-179. [5] Sundaresan A, Risin D, Pellis N.R. Modeled microgravity induced protein kinase C isoform expression in human lymphocytes. J Appl Physiol, 2004, 96(6):2028-2033. [6] Boonyaratanakornkit JB, Cogoli A, Li CF, et al. Key gravity-sensitive signaling pathways drive T-cell activation. FASEB J. 2005, 19: 2020-2042.
- Abstract document
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