Caenorhabditis elegans progeny and Survival under Simulated Microgravity
- Paper number
IAC-12,A1,8,11,x15583
- Author
Prof.Dr. Fawzia Abdel_Rahman, Texas Southern University, United States
- Year
2012
- Abstract
The wild type N2 of the nematode C. elegans was used to test the effect of simulated microgravity on the brood size and survival. C. elegans cultures are maintained in the laboratory on NGM plates seeded with E. coli OP50 as a food source. C. elegans populations in liquid media (S medium) were exposed to simulated microgravity using the NASA recommended system the HARV (High Aspect Ratio Vessels) for different time intervals. Nematodes kept on a shaker served as the control. When each treatment was terminated, the total population was determined to compare it with the initial population, several L4 stages were isolated separately and their progeny was counted. Eggs were extracted from adults to determine the effect of microgravity on hatching of L1. Result showed that simulated microgravity affected the reproduction, survival and the progeny of the exposed C. elegans populations. Simulated microgravity decreased the rate of the reproduction and the progeny, and also increased the mortality of the exposed populations.
- Abstract document
- Manuscript document
(absent)