activation of t cell subset is inhibited after a pre-exposure to modeled microgravity at resting state in an exposure-time dependent manner
- Paper number
IAC-13,A1,7,11,x19729
- Author
Dr. Haiying LUO, China
- Coauthor
Prof. Yong ZHAO, China
- Year
2013
- Abstract
Decline immune function is well documented during and after spaceflights. Microgravity is one of the key factors directly suppressing the function of immune system. T lymphocyte activation plays an important role on immune response. Studies have demonstrated that T cell response to stimuli was inhibited when cultured in real microgravity or a ground based model system. Circulating T cells are, however, maintained in a resting state and their growth and differentiation are strictly regulated under normal conditions. It is not clearly whether activation would be inhibited after a pre-exposure of microgravity on T lymphocytes at the resting state. Here, we investigated the details by measuring the responses of resting T cells to concanavalin A (ConA) after 0h, 8h,16h and 24h pre-exposure of modeled microgravity (MMg) using a rotary bioreactor system. The T cells exhibited depressed activation-marker expression, cytokine secretion and proliferation in response to ConA after the MMg pre-exposure. Most importantly, the cell proliferation was suppressed in an MMg-exposure-time dependant manner, which might be associated, in large partly, with the low expressions of CD25, CD69 and CD71. Moreover, the proliferation of CD4+T cells was more sensitive to the microgravity inhibition than that of CD8+ T cells.
- Abstract document
- Manuscript document
(absent)