Ionizing radiation-induced EMT and transformation in a human bronchial epithelial cell line

Paper number

IAC-15,A1,5,6,x28123

Author

Dr. Jinpeng He, Institute of Modern Physics, Chinese Academy of Sciences, China

Coauthor

Dr. Bing Wang, China

Coauthor

Dr. Fang Sun, China

Coauthor

Prof.Dr. Guangming Zhou, Chinese Academy of Sciences, China

Coauthor

Dr. Jufang Wang, Institute of Modern Physics, Chinese Academy of Sciences, China

Year

2015

Abstract

Purpose: Lung cancer is the largest potential cancer risk in astronauts, howerver, there are few adequate assessment system till now. The purpose of this research is to investigate the celluar and molecular processes involved in lung cancer genesis induced by ionizing radiation exposure. 
Methods: A nontumorigenic human bronchial epithelial cell NL20 was exposed to 2 Gy of X-rays or 1 Gy of carbon ions. The morphology was observed and the survival of the irradiated cells was determined by a clongecic survival assay. The ability to form coloneis in soft agar was used as a biomarker of radiation induced malignat transformation in vitro. The mRNA and protein expression levels of epitelial to mesenchymal transition (EMT) related genes were detected to study the celluler response to ionizing raditon by using qRT-PCR and immunofluorescence analysis, respectively.
Results: NL20 cells exhibited a characteristic epithelial morpholoy while lost that and acquired mesenchymal traits after X-ray or carbon ion radiation exposure. Moreover, the mRNA and protein levels of E-cadherin were both reduced while Vimentin and $\beta$-Catenin were increased. Notably, the efficiency of carbon ions was higher than X-rays. NL20 cells at passage 40 post irradiation proliferated more quickly and the soft agar colony formation efficiency was elevated significantly.
Conclusions: These results suggest that ionizing radiation prometed the development of EMT and neoplastic tranformation in NL20 cells.

Abstract document

IAC-15,A1,5,6,x28123.brief.pdf

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