Structure of the cortical cytoskeleton of cardiomyocytes of mice after 30-day 2g-centrifugation
- Paper number
IAC-15,A1,IP,21,x29759
- Author
Dr. Irina Ogneva, SSC RF Institute of bio-medical problems RAS, Russian Federation
- Coauthor
Mr. Vasily Gnyubkin, France
- Coauthor
Mr. Norbert Laroche, France
- Coauthor
Ms. Maria Maximova, Russian Federation
- Coauthor
Mrs. Irina Larina, Institute for Biomedical Problems, Russian Federation
- Coauthor
Dr. Laurence Vico, France
- Year
2015
- Abstract
Altered external mechanical loading during spaceflights causes negative effects on muscular and cardiovascular systems. We tested the hypothesis that the different isoforms of alpha-actinins dissociate from cortical cytoskeleton under increased/decreased of mechanical load. The state of the cortical cytoskeleton in C57BL6J mice left ventricle cardiomyocytes was investigated after 30-day 2g-centrifugation (“2g” group) and within 12 hours after its completion (“2g+12h” group). We used atomic force microscopy for estimating cell’s transverse stiffness, western-blotting for measuring protein content, and RT-PCR – for estimating their expression level. The transverse stiffness significantly decreased in cardiomyocytes (by 16 percent) in animals of “2g” group (as compared to the control group). In “2g+12h”group, the transverse stiffness significantly decreased in all investigated cell types (in comparison with the relevant values observed in “2g” group). For cardiomyocytes we found that in “2g+12h”group alpha-actinin-1 content decreased in the membranous fraction (by 27 percent) and increased in cytoplasmic fraction (by 28 percent) of proteins (in comparison with the levels in “2g” group). This study has received financial support from the Russian Foundation of the Basic Research (RFBR grant 13-04-00755-a) and from CNES (French Space Agency).
- Abstract document
- Manuscript document
(absent)