Breeding by space-induced mutation and screening of selenium-enriched functional yeast
- Paper number
IAC-17,A1,IP,23,x41015
- Author
Dr. Peng Lei, Fullarton Bio-Tech (Beijing) Co., Ltd., China
- Coauthor
Dr. Bing Huang, Fullarton Bio-Tech (Beijing) Co., Ltd., China
- Coauthor
Prof. Tiehua Zhang, Fullarton Bio-Tech (Beijing) Co., Ltd., China
- Coauthor
Prof. Hongxing Zhang, Fullarton Bio-Tech (Beijing) Co., Ltd., China
- Coauthor
Prof. Hui Liu, Fullarton Bio-Tech (Beijing) Co., Ltd., China
- Coauthor
Prof. Hongwei Hao, Fullarton Bio-Tech (Beijing) Co., Ltd., China
- Year
2017
- Abstract
Space mutation breeding is a new type of microbial mutation breeding technology developed rapidly in recent years, and it refers to the technology of sending the microorganism provided with industrial application value into space by a recoverable satellite or spacecraft. In the space environment of strong-radiation, microgravity, high-vacuum and alternating magnetic field and so on, microorganisms may undergo beneficial or detrimental mutation. After landing to the ground for breeding, new varieties and heritable characteristics are screened to select mutated microorganisms to breed strains with higher quality and yields. Selenium-enriched yeast is an excellent, safe and harmless fermentation carrier. And it is used as good functional foods raw material, with antioxidant, antagonism heavy metals, protecting the cardiovascular system, reducing the carcinogenic rate, assisting the treatment of cancer and so on. Meanwhile, selenium-enriched yeast is also widely used in the field of medicine, health care products and animal feed, while existing in some problems of low selenium enrichment efficiency, low production efficiency and low bioavailability and so on. We have utilized the technology of space mutation to breed and screen high-efficiency selenium-enriched, high production efficiency and high bioavailability yeast mutated strains, and their fermentation performance are optimized to develop and be converted into various kinds of products. Saccharomyces cerevisiae (Fullarton subspecies isolated from Australia) used in industry was exposed to space environment by the Shenzhou-11 and Tiangong-2 spacecraft. After landing to the ground, the yeast strains were screened by gradient resistant plates of sodium selenite (Na2SeO3), and the mutated strains for high-efficiency selenium-enriched were further to optimize to increase production of organic selenium. Now, we have found 5 mutant strains with special features from the recovery spaceflight samples of S. cerevisiae, and they can grow and adapt in the YPD agar and liquid culture of selenium concentration up to 1000mg/L. Meanwhile, the single colony diameter was more than 12mm in the 100mg/L YPD agar plate of selenium concentration, with milk white and margin smoothly and moistly. And we also found the mutated strains can transform the inorganic selenium into organic selenium in the Se concentration of 100mg/L efficiently, the less inorganic selenium accumulated in the yeast cells as compared to the ground control strains. A strain No.F2-26 was selected to optimize its fermentation performance and selenium enrichment efficiency. Our results showed that space mutation breeding is an effective and high-efficiency method for breeding of industrial microorganism.
- Abstract document
- Manuscript document
(absent)