shaken not stirred: comparative studies using 2D-clinostat and random positioning machine

Paper number

IAC-17,A2,7,7,x37936

Author

Dr. Sonja Brungs, German Aerospace Center (DLR), Germany

Coauthor

Dr. Jens Hauslage, German Aerospace Center (DLR), Germany

Coauthor

Mr. Volkan Cevik, German Aerospace Center (DLR), Germany

Coauthor

Dr. Ruth Hemmersbach, German Aerospace Center (DLR), Germany

Year

2017

Abstract

\begin{document}
In the ESA ground-based facility program, clinostats and Random Positioning
Machines (RPM) have been applied to simulated microgravity, while
centrifuges served as a hypergravity model. First results of cell-based
experiments have been recently published{*}. The results clearly demonstrate
that device-specific operational modes have to be considered and discussed
with respect to the induction of shear forces and in turn their usability
as microgravity simulators: In a RPM samples are rotated around two
axes with random velocities and directions, while clinostats apply
constant rotation around one axis.

We will present examples of current investigations visualizing cellular
shear forces within a RPM and a clinostat using Dinoflagellates as
well as macrophages as cellular reporter systems. The latter has already
been studied under real microgravity conditions. We conclude that
one axis clinorotation induces substantially less shear forces in
comparison to random positioning. Therefore, fast-rotating clinostats
provide more valid information and should be preferred for the simulation
of microgravity in cellular experiments.

We believe that this work provides important new insight on the use
and limitations of ground-based facilities for the scientific community,
which will be crucial in the upcoming post-ISS scenario. 

\medskip{}


{*}Topical Issue: Ground-Based Facilities (GBF): Results and Experiences
from ESA\textquoteright{}s Ground-Based Facilities Programme in Space
Life Sciences, eds. Hemmersbach, R., Ngo-Anh, J., Zell, M., Microgravity
Science and Technology, ISSN 0938-0108 Volume 28, Issue 3, June 2016 
\end{document}

Abstract document

IAC-17,A2,7,7,x37936.brief.pdf

Manuscript document

IAC-17,A2,7,7,x37936.pdf (🔒 authorized access only).

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