Differential gene expression patterns induced by parabolic flight and low-shear modelled microgravity in Saccharomyces cerevisiae

Paper number

IAC-19,A1,8,4,x54456

Author

Mr. Sean Farley, Canada, University of Victoria

Coauthor

Ms. Annaliese Meyer, Canada, University of Victoria

Coauthor

Mr. Sean Waugh, Canada, University of Victoria

Coauthor

Ms. Sarah Ebert, Canada, University of Victoria

Coauthor

Prof. Corey Nislow, Canada, University of British Columbia

Coauthor

Dr. Christopher Nelson, Canada, University of Victoria

Coauthor

Mr. Sachintha Premathilaka, Canada, University of Victoria

Coauthor

Mr. Eric Fraser, Canada, University of Victoria

Coauthor

Ms. Cheyenne Heenan, Canada, University of Victoria

Coauthor

Ms. Shannon Dawson, Canada, University of Victoria

Year

2019

Abstract

\begin{document}

Microgravity is a unique environment with profound effects on biological systems. Understanding these effects presents the opportunity to harness and apply them to applications both for spaceflight and for biomedical techniques on earth. Previous investigations have shown that altered mechanical conditions and gravitational forces translate to changes in gene expression, including changes in heat shock proteins and shear stress response. The robust model microorganism \textit{Saccharomyces cerevisiae} is an ideal candidate to uncover mechanisms of responses of interest to a microgravity environment. Access to space is limited by cost and infrequent launches, but ground-based and sub-orbital analogs provide valuable platforms for studies with microgravity conditions. Here we use RNA sequencing to study the changes in gene expression patterns of the model microorganism \textit{S. cerevisiae} in two microgravity conditions. \textit{S. cerevisiae} will be exposed to short-term microgravity onboard a parabolic flight and to low-shear modelled microgravity with a rotating wall vessel. The flight campaign is scheduled for July 22-26th, 2019, on the Canadian Space Agency Falcon-20 parabolic flight aircraft. 

\end{document}

Abstract document

IAC-19,A1,8,4,x54456.brief.pdf

Manuscript document

(absent)