Effects of different hypergravity on platelet functions and thrombus formation
- Paper number
IAC-13,A1,P,73.p1,x16951
- Author
Mr. Guanglei Liu, Beihang University, China
- Coauthor
Dr. Zhicheng Wang, China
- Coauthor
Dr. Suping Li, China
- Coauthor
Mr. Quanwei Shi, China
- Coauthor
Dr. Rong Yan, China
- Coauthor
Prof. Yuedan Wang, Peking University, China
- Coauthor
Prof. Yinghui Li, China Astronaut Research and Training Center, China
- Coauthor
Prof. Yanqiang Bai, Astronaut Center of China, China
- Coauthor
Prof. Kesheng Dai, China
- Year
2013
- Abstract
{\bf Purpose:} Exposure to high gravity environments during short- or long-duration space missions or high-{\it g} training profiles has important medical and health implications for astronauts and pilots. We have recently reported that platelet functions were elevated under hypergravity, however, it is still unclear whether high-{\it g} is strong enough to activate platelets. The aim of the current study is to investigate whether high-{\it g} is strong enough to activate platelets leading to platelet thrombus formation, and the effects of different hypergravity on {\it in vivo} haemostasis. {\bf Methods:} Adult C57BL/6 black mice were anesthetized and subjected to various levels of hypergravity or 1 {\it g} as synchronous group. Thrombus formation was detected by immunohistochemistry analysis and the states of circulating platelets of mice were tested by tail-bleeding time assay and circulating platelet aggregates. P-selectin surface expression of platelets from treated mice was measured by Flow cytometry. {\bf Results:} The tail-bleeding time was significantly shortened in mice exposed to 12 {\it g} for 20 minutes compared with the synchronous 1 {\it g} controls. Immunohistochemistry analysis showed that platelet thrombi were formed in ventricle or blood vessels of the heart, or brain and lung from 12 {\it g}-exposed mice. P-selectin surface expression was significantly enhanced in platelets from 12 {\it g}-exposed mice compared with 1 {\it g} controls. The tail-bleeding time and the ratio of circulating platelet aggregates of mice exposed to 2 {\it g} for 20 minutes, 4 {\it g} for 10 minutes, or 8 {\it g} for 10 minutes, corresponding to hypergravity inflicted on astronauts during space missions, were significantly reduced. However, there was no obvious difference in tail-bleeding time between the 1 {\it g} controls and the mice exposed to 6 {\it g} for 80 seconds or 10 {\it g} for 60 seconds corresponding to some high-{\it g} training profiles. {\bf Conclusions:} These results indicate that platelets could be activated by hypergravity leading to platelet thrombus formation {\it in vivo}. The high gravity during some launch and re-entry is strong enough to elevate platelet functions, whereas, the high-{\it g} training profiles are relatively safe for most of the astronauts and pilots. These researches reveal the pathogenesis of gravity-change-related hemorrhagic and thrombotic diseases, and also suggest that the special attention should be paid to these kinds of diseases under different levels of gravity.- Abstract document
- Manuscript document
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