the mechanism of astrocyte’s resistant to rays
- Paper number
IAC-13,A1,P,27.p1,x19416
- Author
Prof.Dr. Yulin Deng, China
- Coauthor
Dr. Tuo Zhao, China
- Coauthor
Dr. Hailong Wang, China
- Coauthor
Prof. Hong Ma, China
- Year
2013
- Abstract
purpose: the purpose of this study was to investigate the mechanism of glioma’s resistant to γ-rays. Methods: Glioma was radiated by γ-ray(7Gy) and the cell cycle was evaluated by flow cytometry and the samples’ genes related to DNA replication and DNA repair were analyzed after genomic sequencing. Results: the proportion of cells in S-phase was increased compared to control group. The expression of genes involved in DNA replication and DNA repair(homologous recombination repair, non-homologous end joining (NHEJ) and mismatch repair ) decreased while genes related to Base excision repair increased. Conclusion: γ-rays would induce a single-strand break and base mutation. U251 would be blocked in S-phase probably by the way of inhibting DNA replication. Broken DNA was repaired by the way of base mismatch repair and single-strand repair.
- Abstract document
- Manuscript document
(absent)